Journal: Current Research in Pharmacology and Drug Discovery

Article Title: Apigenin attenuates depressive-like behavior via modulating monoamine oxidase A enzyme activity in chronically stressed mice

doi: 10.1016/j.crphar.2023.100161

Figure Lengend Snippet: Apigenin inhibited monoamine oxidase A inhibitory enzymatic activity in the hippocampal brain regions and prefrontal cortex of CUMS-induced mice (A) Inhibitory effect of apigenin on the expressions of MAOA immunoreactive positive cells in the CA1, CA3, dentate gyrus and prefrontal cortex of CUMS-induced mice after immunohistochemistry (B) Inhibitory effect of apigenin on the density of MAOA immunoreactive positive cells in the CA1 of CUMS-induced mice (C) Inhibitory effect of apigenin on the density of MAOA immunoreactive positive cells in the CA3 of CUMS-induced mice (D) Inhibitory effect of apigenin on the density of MAOA immunoreactive positive cells in the dentate gyrus of CUMS-induced mice (E) Inhibitory effect of apigenin on the density of MAOA immunoreactive positive cells in the prefrontal cortex of CUMS-induced mice. Scale bar = 320 μm (n = 10, ***P < 0.001, ****P < 0.0001 v control; **P < 0.01, ****P < 0.0001 v stress). Data expressed as mean ± SD. One-way analysis of variance (ANOVA) followed by Tukey's multiple comparison test.

Article Snippet: The expressions of positive cells of MAOA in mice brains were estimated using immunohistochemistry kit (Elabscience, USA with Cat. No: E-AB-60293 MAOA Polyclonal antibody) in conformity with the manufacturer's instructions.

Techniques: Activity Assay, Immunohistochemistry, Control, Comparison

Journal: Neuroscience

Article Title: MicroRNA-132 dysregulation in Toxoplasma gondii infection has implications for dopamine signaling pathway

doi: 10.1016/j.neuroscience.2014.03.015

Figure Lengend Snippet: Protein expression of (A) DRD1; (C) DRD5; (E) MAOA; (I) DARPP-32 Phosphorylation at Ser97; and (K) DARPP-32 Phosphorylation at Thr34 between control and infected mice. Quantification of normalized level of (B) DRD1; (D) DRD5; (F) MAOA; (J) DARPP-32 Phosphorylation at Ser97; and (L) DARPP-32 Phosphorylation at Thr34 between control and infected mice. (G) Total DARPP-32 expression is comparable between control and infected mice. (H) Quantification of normalized level of DARPP-32 shown in (G). Protein levels were compared to β-actin. Control mice: n = 6 - 7; Infected mice: n = 6 - 8; Error bars = 1 SD. * < 0.05, ** < 0.01.

Article Snippet: The striatum of mouse brain was homogenized in RIPA buffer (Sigma) containing protease and phosphatase inhibitors, sonicated at 4 °C for 5 min, and centrifuged at 10,000 g for 5 min. Proteins were probed with primary antibodies for DRD1 (#sc-14001, 1:500), DRD5 (#sc-25650, 1:500) from Santa Cruz, MAOA (#B02P, 1:500, Abnova), DARPP-32 (#2302, 1:2000), Phospho-DARPP-32 at Ser97 (#3401, 1:2000), at Thr34 (#5393, 1:2000), at Thr75 (#2301, 1:2000) from Cell Signaling Technology.

Techniques: Expressing, Phospho-proteomics, Control, Infection

Journal: Heliyon

Article Title: Mapping the 5-HTergic neural pathways in perimenopausal mice and elucidating the role of oestrogen receptors in 5-HT neurotransmission

doi: 10.1016/j.heliyon.2024.e27976

Figure Lengend Snippet: Primary antibodies used in this study.

Article Snippet: Rabbit anti-mouse MAOA antibody , A1354 , ABclonal , 1:1000 (WB).

Techniques:

Journal: Journal of neural transmission (Vienna, Austria : 1996)

Article Title: Abnormalities of neurotransmitter and neuropeptide systems in human neuroepithelioma cells infected by three Toxoplasma strains

doi: 10.1007/s00702-013-1064-3

Figure Lengend Snippet: Antibodies used for validation by western blot analysis

Article Snippet: MAOA polyclonal antibody , Abnova , 1:500 , anti-rabbit.

Techniques: Biomarker Discovery, Western Blot

Journal: Journal of neural transmission (Vienna, Austria : 1996)

Article Title: Abnormalities of neurotransmitter and neuropeptide systems in human neuroepithelioma cells infected by three Toxoplasma strains

doi: 10.1007/s00702-013-1064-3

Figure Lengend Snippet: (A) to (G), Toxoplasma type I elicits a strain-dependent changes on proteins of GRIN2A (150-250 kDa), HTR3E (∼50 kDa), DRD1 (60-80 kDa), PROK2 (20-25 kDa), TAC1 (20-25 kDa), SLC1A3 (50-75 kDa), and MAOA (∼60 kDa); (H) type III elicits a strain-dependent change on TDO2 (∼50 kDa); β-actin immunoreactivity (42 kDa) is displayed as loading controls. (I) Levels of proteins were calculated by densitometric analysis of the immunoreactive band. Each protein was compared to β-actin and normalized to untreated. Data, expressed as percent of control, are the mean + SD of three separate preparations of infected cells. * P < 0.05; ** p < 0.01 versus control.

Article Snippet: MAOA polyclonal antibody , Abnova , 1:500 , anti-rabbit.

Techniques: Control, Infection

Journal: Journal of neural transmission (Vienna, Austria : 1996)

Article Title: Abnormalities of neurotransmitter and neuropeptide systems in human neuroepithelioma cells infected by three Toxoplasma strains

doi: 10.1007/s00702-013-1064-3

Figure Lengend Snippet: Differentially expressed genes involved in the pathway of neurotransmitters and neuroactive molecules

Article Snippet: MAOA polyclonal antibody , Abnova , 1:500 , anti-rabbit.

Techniques: Microarray